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目的:探讨稳心草黄酮类化合物对肺动脉平滑肌细胞增殖的影响。方法:采用四氮唑蓝比色法(MTT)测定细胞增殖、双抗体夹心ABC-El i sa法测定细胞外血小板源生长因子(PDGF)含量的变化。结果:稳心草黄酮类化合物具有抑制肺动脉平滑肌细胞的增殖及下调细胞外PDGF水平的作用,与对照组相比有显著性差异(P<0.05)。结论:稳心草黄酮类化合物可显著抑制肺动脉平滑肌细胞的增殖。
Objective: To investigate the effects of flavonoids from Brassica on proliferation of pulmonary artery smooth muscle cells. Methods: Cell proliferation was measured by MTT assay. The change of extracellular platelet derived growth factor (PDGF) content was detected by double antibody sandwich ABC-Elisa method. Results: Flavonoids could inhibit the proliferation of pulmonary artery smooth muscle cells and down-regulate the level of extracellular PDGF, which was significantly different from the control group (P <0.05). CONCLUSION: Coptis flavonoids can significantly inhibit the proliferation of pulmonary artery smooth muscle cells.