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为了观察猴艾滋病D型逆转录病毒Ⅰ型(SRV1)感染和未感染的Raji细胞同工酶活性的变化,用超声波破碎细胞,提取细胞内总蛋白,然后用垂直薄层聚丙烯酰胺凝胶等电聚焦电泳对其进行酶谱分析。 以α-醋酸萘酯和/或β-醋酸萘酯为底物时,SRV1感染的Raji细胞近酸性端酯酶Est 11-14的活性是未感染Raji细胞的2.2倍,提示酯酶活性的变化与病毒感染有关。SRV1感染和未感染Raji细胞系的酸性磷酸酶的酶带数,pI值和酶活性无差异,二者均没有检出乳酸脱氢酶(LDH)、醇脱氢酶(ADH)、过氧化物酶(POD)、苹果酸脱氢酶(MDH),但有较弱的碱性磷酸酶(APH)。这些结果提示酯酶活性的增加有可能作为SRV1感染Raji细胞的一种指标。
In order to observe the changes of Raji cell isozymes in monkeys infected with retrovirus type A (SRV1) of monkey AIDS, the cells were disrupted by sonication and the total cellular protein was extracted. Electrofocusing electrophoresis was used to perform zymogram analysis. When using α-naphthyl acetate and / or β-naphthyl acetate as substrates, the activity of the near acidic terminal esterase Est 11-14 of Raji cells infected with SRV1 was 2.2-fold higher than that of uninfected Raji cells, suggesting a change in esterase activity And the virus infection. There was no difference in the number of acid phosphatase, pI and enzyme activity between SRV1 infected and uninfected Raji cell lines, and neither lactate dehydrogenase (LDH) nor alcohol dehydrogenase (ADH), peroxide Enzyme (POD), malate dehydrogenase (MDH), but weaker alkaline phosphatase (APH). These results suggest that increased esterase activity may serve as an indicator of SRV1-infected Raji cells.