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目的：观察不同月龄小鼠帕金森病（PD）模型黑质纹状体系统氧化应激损伤的增龄性改变并检测老龄PD小鼠氧化应激相关基因的差异性表达。方法选用健康雌性3、6、10月龄快速老化小鼠P8系（SAMP8）42只，各月龄小鼠随机平均分为 MPTP 组与对照组，分别给予背部皮下急性注射1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）及等量0．9％NaCl处理。给药后72 h，采用开放旷场实验观察其运动功能，高效液相色谱法检测黑质DA含量，分光光度计法检测纹状体超氧化物歧化酶（SOD1）活性和丙二醛（MDA）含量，比较不同月龄小鼠黑质DA系统、纹状体氧化应激相关指标损伤的差异。采用PCR Array检测两组10月龄小鼠纹状体氧化应激相关基因表达的差异。结果与对照组相比，MPTP组各月龄小鼠水平运动距离与站立次数均减少，DA水平、SOD活性明显下降，MDA含量明显增加（P＜0．05）；与3、6月龄相比，10月龄小鼠上述指标变化更明显；与对照组相比，10月龄MPTP组小鼠环氧化酶-2表达明显上调，而谷胱甘肽过氧化物酶3、6、8，乳酸过氧化物酶、核氧化还原酶、肌红蛋白、神经珠蛋白酶、过氧化物还原酶1和嗜酸粒细胞过氧化物酶9种基因明显下调（倍数改变＞2）。结论月龄是影响PD模型黑质纹状体系统损伤的重要因素；与氧化应激相关的基因的上调或下调可能参与了PD的早期发病。“,”Objective To observe the age-related changes of nigro-strital oxidative stress in a mice model of Parkinsons disease(PD)at different ages and detect the differential expression of oxidative stress related genes in aged PD mice by using PCR Array.Methods Forty-two healthy female senescence-accelerated mouse prone 8 (SAMP8)mice aged 3, 6 and 10-month were averagely and randomly divided into 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) group and control group,which were subcutaneously injected with MPTP or the same volum of 0.9%NaCl,respective-ly.After the first injection for 72 hours,behavioral changes in mice were examined by the open field test.The levels of dopamine (DA)in nigro-striatal system was measured by a high performance liquid chromatography with electochemi-cal detector (HPLC-ECD).The activities of superoxide dismutase 1 (SOD1 )and the content of malondialdehyde (MDA)were detected by the spectrophotometer.The injuries of nigraldopamine system and striatal oxidative stress re-lated indexes were compared among mice at different ages.The expression of striatal oxidative stress related gene ex-pression in 10-month mice was detected by PCR Array.Results Compared with control group,the levels of DA and SOD 1 ,the performance in the open field test all decreased in MPTP group at three ages,while the content of MDA in tissue remarkably increased (P<0.01 ).Moreover,the above changes in 10-month mice were more obvious than 3-and 6-month mice (P2 ).Conclusion Aging plays an important role in nigro-striatal system injury of PD model.Up-or down-regulation of oxidative stress related genes may participate in the early phase of PD.