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本实验每日以5mg/kg体重3-氯丙二醇和75mg/kg体重2,3-氧丙醇合并用药给雄大鼠灌胃,连续2天,停药8天,以10天为一疗程,共给药9疗程。于给药3、6、9疗程及3、6疗程停药后90天,9疗程停药后120天,分别对大鼠睾丸、附睾的组织学改变以及内脏器官组织学变化、末梢血液细胞计数及分类、骨髓细胞增生度及骨髓细胞微核率进行检查。研究结果表明,合并用药引起睾丸生精上皮功能抑制和附睾内精子退变所产生的抗生育作用是可逆的;对造血功能以及内脏的组织学结构、骨髓细胞微核率的影响不明显,或无统计学意义。因此,以小剂量3-氯丙二醇和2,3-氧丙醇间歇(疗程)长期给药,无明显毒性和副作用。
In this experiment, male rats were fed with 5 mg/kg body weight of 3-chloropropanediol and 75 mg/kg body weight of 2,3-oxopropanol daily for 2 days, with 8 days of discontinuation, and 10 days as a course of treatment. Dosing 9 courses. During the 3,6,9 courses of drug administration and 90 days after discontinuation of 3,6 treatment courses, 120 days after discontinuation of 9 treatment courses, the histological changes of the testes and epididymis of the rats, histological changes of the internal organs, and peripheral blood cell counts were separately performed. And classification, bone marrow cell proliferation and bone marrow cell micronucleus rate were examined. The results of the study indicate that the combined use of drugs causes reversible testicular spermatogenic inhibition and anti-fertility effects of epididymal sperm degeneration; the effect on hematopoietic function and the visceral histological structure and bone marrow cell micronucleus rate is not significant, or No statistical significance. Therefore, long-term administration of small doses of 3-chloropropanediol and 2,3-oxopropanol intermittently (courses) did not have significant toxicity and side effects.