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目的建立宜肝乐颗粒的定性、定量鉴别方法。方法采用薄层色谱法对宜肝乐颗粒中鸡矢藤、功劳木、虎杖进行定性鉴别;高效液相色谱法测定宜肝乐颗粒中大黄素,Zorbax C18色谱柱(150 mm×4.6 mm,5μm);流动相:甲醇-0.1%磷酸溶液(80∶20);检测波长:288 nm;体积流量:1.0 mL/min;柱温:室温;进样量:10μL。在此色谱条件下,理论塔板数按大黄素峰计达3 000以上,分离度为2.0。结果3味药材的薄层色谱鉴别呈阳性;建立的定量测定方法可用于宜肝乐颗粒中大黄素的测定。结论薄层色谱法和高效液相色谱法简便、准确、灵敏,可作为宜肝乐颗粒质量控制的有效方法。
Objective To establish a qualitative and quantitative method for identification of Yiganle Granules. METHODS: TLC was used to qualitatively identify the chicken vector of Rhizoma sagittifolia, G. sinensis, and Polygonum cuspidatum in Yiganle granules. HPLC was used to determine emodin in Yiganle granules and Zorbax C18 column (150 mm×4.6 mm, 5 μm). Mobile phase: methanol-0.1% phosphoric acid solution (80:20); Detection wavelength: 288 nm; Volume flow rate: 1.0 mL/min; Column temperature: room temperature; Injection volume: 10 μL. Under this chromatographic condition, the number of theoretical plates is more than 3,000 based on emodin peaks, and the degree of separation is 2.0. [Results] TLC was positive for the identification of 3 herbs. The established quantitative method was used for the determination of emodin in Yigan Le Granules. Conclusion TLC and HPLC are simple, accurate, and sensitive, and can be used as an effective method for quality control of Yigan Le Granules.