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目的:观察黄精多糖(Polygonatum sibiricum polysaccharide,PSP)对顺铂(cisplatin,CP)致大鼠肝损害的保护作用及抗氧化指标的影响。方法:采用ip顺铂5 mg.kg-1的方法制备肝损害模型,将40只大鼠随机分为5组:空白组、模型组、PSP低、中、高剂量组(10,20,40 g.kg-1);分别ig处理10 d,采血检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平;处死后剪取肝测定肝脏系数,肝组织匀浆后检测超氧化物歧化酶(SOD)、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-Px)含量;同时观察肝脏组织学结构变化。结果:与空白组比较,模型组大鼠血清ALT,AST含量均显著升高(P<0.01),肝组织匀浆中SOD,GSH-Px均下降(P<0.01),而MDA含量升高(P<0.01)。与模型组比较,PSP作用后大鼠血清ALT,AST含量均明显降低(P<0.05或P<0.01),肝组织匀浆中SOD,GSH-Px均明显升高(P<0.05或P<0.01),MDA含量明显下降(P<0.05或P<0.01)。结论:黄精多糖可明显降低顺铂致大鼠肝功能损伤,其机制可能与其抗氧化作用有关。
Objective: To observe the protective effect of Polygonatum sibiricum polysaccharide (PSP) on hepatic injury induced by cisplatin (CP) in rats and the effect of anti-oxidation. Methods: Liver damage model was established by ip cisplatin 5 mg.kg-1. 40 rats were randomly divided into 5 groups: blank group, model group, PSP low, medium and high dose groups (10, 20, 40 g.kg-1). The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured by ig for 10 days. The liver samples were harvested and the hepatic coefficient was measured. After liver homogenate, (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were measured. The histological changes of liver were also observed. Results: Compared with the blank group, the contents of ALT and AST in the model group were significantly increased (P <0.01), the content of SOD and GSH-Px in the homogenate of the model group were decreased (P <0.01) P <0.01). Compared with model group, serum ALT and AST levels were significantly decreased (P <0.05 or P <0.01) and SOD and GSH-Px in liver homogenate were significantly increased (P <0.05 or P <0.01) ), MDA content decreased significantly (P <0.05 or P <0.01). Conclusion: Polygonatum polysaccharides can significantly reduce the cisplatin-induced liver injury in rats, its mechanism may be related to its antioxidant effect.