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目的探讨淫羊藿总黄酮对心力衰竭(心衰)大鼠心肌一氧化氮(NO)的影响。方法雄性SD大鼠100只,随机分为两组:空白组(n=10);模型组(n=90只)皮下注射异丙基肾上腺素2次(170mg/kg),6周后应用超声心动图检测,以左室射血分数(LVEF)≤45%确定造模成功,将模型分为心衰代偿组、心衰失代偿组,再将心衰失代偿组随机分为心衰对照组、美托洛尔组、淫羊藿总黄酮组(大、中、小剂量),18周后使用左心导管进行血流动力学检查,利用硝酸还原酶法检测心肌NO浓度,并取心肌观察病理形态学特征。结果与空白组相比,心衰代偿和心衰对照组心功能指标显著恶化,心肌NO、心室重量指数明显升高(P<0.01);与心衰对照组相比,淫羊藿总黄酮各剂量组血流动力学指标显著好转,心肌NO、心室重量指数明显降低(P<0.01)。结论淫羊藿总黄酮能够改善实验性心力衰竭大鼠心功能,降低心肌NO水平。
Objective To investigate the effect of Epimedium Flavonoids on myocardial nitric oxide (NO) in heart failure (CHF) rats. Methods 100 male Sprague-Dawley rats were randomly divided into two groups: blank group (n = 10); model group (n = 90) subcutaneous injection of isoproterenol twice (170mg / kg) Cardiogram detection, left ventricular ejection fraction (LVEF) ≤ 45% to determine the success of modeling, the model is divided into heart failure compensation group, heart failure decompensation group, and then decompensated heart failure group were randomly divided into heart The rats in the control group, metoprolol group and epimedium flavone group (large, medium and small doses) were subjected to left heart catheterization for hemodynamics 18 weeks later, NO concentration in myocardium was detected by nitrate reductase method Take the myocardium to observe the pathomorphological characteristics. Results Compared with the blank group, heart failure index and heart failure in the control group significantly decreased cardiac function index, myocardial NO, ventricular mass index was significantly increased (P <0.01); Compared with the heart failure control group, Epimedium Flavonoids The indexes of hemodynamics were significantly improved in each dose group, and the myocardial NO and ventricular weight index were significantly decreased (P <0.01). Conclusion Epimedium Flavonoids can improve cardiac function and reduce myocardial NO level in experimental heart failure rats.