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目的:探讨绞股蓝总皂甙最佳的提取方法。方法:首先选用水、80%甲醇、75%乙醇和氯仿等作为溶剂提取绞股蓝总皂甙,从中选择出2种较优试剂,再以回流提取法、索氏提取法、超声波法等提取方法对绞股蓝的总皂甙进行提取,从而选出一种优良的提取方法。并且采用分光光度法测定了绞股蓝总皂甙的含量。结果:研究表明,75%乙醇为绞股蓝总皂甙提取的最佳溶剂,乙醇回流提取法为最优方法。结论:以75%乙醇为提取溶剂的回流提取法是一种高效、简便、节能的绞股蓝总皂甙的提取方法。
Objective: To explore the best extraction method of Gynostemma saponins. Methods: Firstly, water, 80% methanol, 75% ethanol and chloroform were used as solvent to extract Gynostemma total saponin. Two kinds of better reagents were selected, and reflux extraction method, Soxhlet extraction method and ultrasonic method were used to extract Gynostemma pentaphyllum. The total saponins are extracted to select an excellent extraction method. Spectrophotometry was used to determine the total saponin content of Gynostemma pentaphyllum. Results: Studies have shown that 75% ethanol is the best solvent for the extraction of Gynostemma saponins, and ethanol reflux extraction is the best method. Conclusion: The reflux extraction method with 75% ethanol as extraction solvent is an efficient, simple, and energy-saving extraction method for total saponins of Gynostemma pentaphyllum.