蝮蛇抗栓酶是从蝮蛇毒中提取的一种酶制剂 ,具有抗凝、溶栓、扩张血管等多种功效 ,尤其在增强纤溶系统活性方面作用更明显。本文作者着眼于抗栓酶抗体的制备 ,利用亲和层析的原理和方法对蝮蛇抗栓酶大量提纯 ,首先获得了产生抗蝮蛇抗栓酶单克隆抗体的杂交瘤 4 E1和 7B3细胞株 , Agkistrodon anthelmintic enzyme is a kind of enzyme preparation extracted from Agkistrodon flavelloviruses. It has many effects such as anticoagulant, thrombolysis and vasodilation, especially in enhancing the activity of fibrinolytic system. In this paper, the authors focus on the preparation of antithrombin antibody, using the principle and method of affinity chromatography for the purification of viper antithrombin, first obtained a hybridoma 4 E1 and 7B3 cells producing anti-viper antithrombin monoclonal antibody Strains,