Objective:To evaluate antioxidant,anti-inflammatory,hepatoprotective and vasorelaxant activities of Pupulus nigra flower buds ethanolic extract.Methods:Antioxidant and anti-inflammatory activities of the extract were assessed using respectively the ABTS test and the animal model of carrageenan-induced paw edema.Protection from hepatic toxicity caused by aluminum was examined by histopathologic analysis of liver sections.Vasorelaxant effect was estimated in endothelium-intact and-nibbed rings of porcine coronary arteries precontracted with high concentration of U466I9.Results:The results showed a moderate antioxidant activity(40%),but potent anti-inflammatory activity(49.9%)on carrageenan-induced mice paw edema,and also as revealed by histopathologic examination,complete protection against AlCl_3-induced hepatic toxicity.Relaxant effects of the same exlracl on vascular preparation from porcine aorta precontracted with high concentration of U466I9 were considerable at 10~(-1)g/L,and comparable(P>0.05)between endothelium-intact(67.74%,IC_(50)0.04 mg/ml.)and-rubbed(72.72%,IC_(50)=0.075 mg/ml,)aortic rings.Conclusions:The extract exerted significant anti-inflammatory,hepatoprotective and vasorelaxant activities,the latter being cndothelium-independent believed to be mediated mainly by the ability of components present in the extract to exert antioxidant properties,probably related to an inhibition of Ca~(2+)influx. Objective: To evaluate antioxidant, anti-inflammatory, hepatoprotective and vasorelaxant activities of Pupulus nigra flower buds ethanolic extract. Methods: Antioxidant and anti-inflammatory activities of the extract were assessed using individually the ABTS test and the animal model of carrageenan-induced paw edema . Protection from hepatic toxicity caused by aluminum was was examined by histopathologic analysis of liver sections. Vasorelaxant effect was estimated in endothelium-intact and-nibbed rings of porcine coronary arteries precontracted with high concentration of U466I9.Results: The results showed a moderate antioxidant activity ( 40%), but potent anti-inflammatory activity (49.9%) on carrageenan-induced mice paw edema, and also as revealed by histopathologic examination, complete protection against AlCl 3-induced hepatic toxicity. Relaxant effects of the same exlracl on vascular preparation from porcine aorta precontracted with high concentration of U466I9 were considerable at 10 ~ (-1) g / L, and comp arable rings between the endothelium-intact (67.74%, IC 50 (50) 0.04 mg / ml) and -rubbed (72.72%, IC 50 = 0.075 mg / ml) significant anti-inflammatory, hepatoprotective and vasorelaxant activities, the latter being cndothelium-independent believed to be mediated primarily by the ability of components present in the extract to exert antioxidant properties, probably related to an inhibition of Ca ~ (2+) influx.