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研究旨在明确长期男子或宫颈因素不育夫妇用洗涤处理的精子宫内授精(IUI-SW)的妊娠率是否高于第一段射精精液宫内授精(IUI-SE)的妊娠率。研究对象为41对40岁以下就诊于Chaim Sheba医疗中心生殖内分泌诊所的不育夫妇,27对为男子不育,14对为宫颈性不育。病人在第一治疗周期随机接受IUI-SE或IUI-WS,第二周期交换方法,第三周期重复第一种方法。用hMG/hCG或CC/hMG/hCG联合诱导滤泡生成和排卵。精液制备:禁欲3天,手淫取精,置无菌器内,液化后混合,取一份作精液分析。剩余精液用Earle氏培养液稀释,500转离心6分钟。弃上清液,小团混悬于新鲜培养
The aim of this study was to determine whether the pregnancy rate of IUI-SW for washing treatment of long-term male or cervical infertile couples is higher than the pregnancy rate of the first intrauterine insemination (IUI-SE). The subjects were 41 pairs of infertile couples under 40 years of age at the Reproductive Endocrinology Clinic at Chaim Sheba Medical Center, 27 were male infertile and 14 were cervical infertility. Patients were randomized to either IUI-SE or IUI-WS during the first treatment cycle, the second cycle of exchange, and the third cycle to repeat the first. Follicular formation and ovulation were induced with hMG / hCG or CC / hMG / hCG. Preparation of semen: 3 days abstinence, masturbation fine, home sterile, liquefied and mixed, take a copy for semen analysis. The remaining semen is diluted with Earle’s medium and centrifuged at 500 rpm for 6 minutes. The supernatant was discarded and the pellets suspended in fresh culture