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目的：研究硒酸酯多糖（ＫＳＣ）对ＣＤ＿３ＡＫ细胞增殖活性和杀瘤活性的增强效应。方法：应用ＣＤ＿３单抗（ＣＤ＿３ＭｃＡｂ）和小剂量ｒＩＬ－２从正常外周血单个核细胞中诱生ＣＤ＿３ＡＫ细胞；观察ＫＳＣ在诱生和扩增阶段对ＣＤ＿３ＡＫ细胞增殖特性（增殖指数、激活率、克隆率和分裂指数）、ＩＬ－２Ｒ表达水平和对Ｒａｊｉ、Ｌ＿（１２１０）细胞杀伤活性的影响。结果：ＫＳＣ（５μｇ／ｍｌ）单独对ＣＤ＿３ＡＫ细胞的增殖无明显作用，但增强ＩＬ－２和ＣＤ＿３ＭｃＡｂ的作用效应，ＣＤ＿３ＡＫ细胞的增殖指数、激活率、克隆率和分裂细胞指数分别提高３９．３％、１２．３％、６３．３％和４９．５％；ＩＬ－２Ｒ表达水平增高９３．５％；杀瘤活性约提高２倍。ＫＳＣ＞１０μｇ／ｍｌ时反呈抑制效应。结论：硒酸酯多糖显著增强ＣＦ＿３ＡＫ细胞的增殖活性和杀瘤活性。
Objective: To study the enhancing effect of selenate polysaccharide (KSC) on the proliferation and tumorigenicity of CD_3AK cells. Methods: CD_3AK cells were induced from normal peripheral blood mononuclear cells by CD_3 McAb (CD_3McAb) and low dose of rIL-2. The proliferation of CD_3AK cells (proliferation index, activation rate, cloning Rate and division index), the level of IL-2R expression and the cytotoxicity on Raji and L 1210 cells. Results: KSC (5μg / ml) alone had no effect on the proliferation of CD_3AK cells, but enhanced the effect of IL-2 and CD_3McAb. The proliferation index, activation rate, clonality rate and dividing cell index of CD_3AK cells increased by 39.3% , 12.3%, 63.3% and 49.5%, respectively. The level of IL-2R was increased by 93.5% and the cytotoxic activity was increased about 2-fold. KSC> 10μg / ml showed anti-inhibitory effect. CONCLUSION: Selenate polysaccharide significantly enhanced the proliferation and tumorigenicity of CF_3AK cells.