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目的：建立类似临床大肠癌根治术后肝转移动物模型，并建立精确评估肝转移的方法。方法：在裸鼠脾内注入人结肠癌细胞5min后切除脾脏。术后第30天对裸鼠行病理检查并测定肝转移DNA含量。结果：发现肝转移率为100％，肝外其它脏器未见转移结节,组织学证实肝转移结节为低分化腺癌。脾内注入105癌细胞、脾切除组肝转移癌DNA的含量比脾内注入106癌细胞、脾切除组肝转移癌DNA的含量低61.4％。结论：本实验方法建立的模型能很好地模拟临床上大肠癌根治术后肝转移征象，肝转移DNA含量的测定具有准确、可靠的优点。
Objective: To establish an animal model of hepatic metastasis similar to clinical radical resection of colon cancer and establish a method for accurate assessment of liver metastases. METHODS: The spleen was excised 5 minutes after injecting human colon cancer cells into the spleen of nude mice. On the 30th day after operation, the nude mice were examined pathologically and DNA content of liver metastases was measured. Results: The liver metastasis rate was found to be 100%. No metastatic nodules were found in other extrahepatic organs. Histologically confirmed liver metastases were poorly differentiated adenocarcinoma. The intraspinal injection of 105 cancer cells and splenectomized liver metastases had a DNA content lower than that of 106 cells injected into the spleen and 61.4% of liver metastatic carcinoma DNA in the splenectomized group. Conclusion: The model established by this experimental method can well simulate the clinical liver metastasis after radical resection of colon cancer. The determination of DNA content in liver metastasis has the advantages of accurate and reliable.