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目的:探讨三七总皂苷预处理对急性内脏痛大鼠的影响,初步阐述三七总皂苷对急性内脏痛的影响机制。方法:成年雌性SD大鼠54只随机分为正常组(n=6),生理盐水预处理组(n=24),三七总皂苷预处理组(n=24)。正常组常规条件饲养,不做干预及建立急性内脏痛模型;生理盐水预处理组和三七总皂苷预处理组大鼠分别预先腹腔注射生理盐水(2.86 ml/kg)或7 mg/ml的三七总皂苷(2.86 ml/kg),每12 h一次,连续7 d,第8天腹腔注射1%乙酸(10 mg/kg),建立急性内脏痛模型,立即观测SD大鼠扭体反应。按(30、60、90、180 min)不同存活时间处死动物,免疫组化法观测脊髓背角GFAP的表达变化。结果:VPI评分显示,三七总皂苷能显著下调内脏痛模型VPI评分,减轻疼痛。免疫组化显示在相同时间点,三七总皂苷预处理组大鼠GFAP表达弱于生理盐水预处理组,尤其在30、60、90 min存活组。结论:对SD大鼠急性内脏痛模型预先腹腔注射三七总皂苷可以抑制脊髓胶质细胞激活,从而减轻急性内脏痛。
Objective: To investigate the effects of Panax notoginseng saponins preconditioning on acute visceral pain in rats, and to elucidate the mechanism of Panax notoginseng saponins on acute visceral pain. Methods: Fifty-four adult female Sprague-Dawley rats were randomly divided into normal group (n = 6), normal saline preconditioning group (n = 24) and panax pseudoginseng total saponin preconditioning group (n = 24). Rats in the normal saline group and the Panax notoginseng saponin preconditioning group were intraperitoneally injected with normal saline (2.86 ml / kg) or 7 mg / ml of tris The total ginsenoside (2.86 ml / kg) was given every 12 hours for 7 days. The rats were injected intraperitoneally with 1% acetic acid (10 mg / kg) on the 8th day to establish acute visceral pain model. Animals were killed at (30,60,90,180 min) different survival time, and the expression of GFAP in spinal dorsal horn was observed by immunohistochemistry. Results: VPI score showed that Panax Notoginseng Saponins could significantly reduce the VPI score of visceral pain model and relieve the pain. Immunohistochemistry showed that GFAP expression in the panax notoginseng saponin pretreatment group was weaker than that in the saline pretreatment group at the same time point, especially at 30, 60 and 90 min. Conclusion: The intraperitoneal injection of Panax notoginseng Saponins can inhibit the activation of spinal cord glia cells and thus reduce acute visceral pain in acute visceral pain model of SD rats.