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目的 :观察益气通络丹 (YQTLD)含药血清对培养SD大鼠血管平滑肌细胞 (VSMC)细胞周期的影响 ,探讨该药抗冠心病、脑血栓等心脑血管疾病的作用机制。方法 :用血清药理学方法制备含药血清 ,用流式细胞术 (FCM )测定作用2 4h后含药血清对VSMC细胞周期的影响情况。结果 :益气通络丹含药血清能使SD大鼠VSMC的G1期细胞所占百分数增加 ,细胞增殖率 (PI)值降低 ,与空白血清对照组有显著性差异 (P <0 .0 1)。结论 :益气通络丹含药血清能阻滞细胞停留在G0G1期 ,抑制细胞进入S期 ,有抗VSMC的增殖作用 ,这可能是其临床上抗冠心病、脑血栓的作用机制之一。
Objective : To observe the effect of serum containing YQTLD on the cell cycle of cultured rat vascular smooth muscle cells (VSMCs), and to explore the mechanism of this drug against cardiovascular and cerebrovascular diseases such as coronary heart disease and cerebral thrombosis. Methods: Serum containing drugs was prepared by serum pharmacology and flow cytometry (FCM) was used to determine the effect of drug-containing serum on the cell cycle of VSMCs after 24 hours. Results: The serum containing Yiqi Tongluodan could increase the percentage of G1 phase cells in the VSMCs of SD rats, and the cell proliferation rate (PI) value decreased, which was significantly different from the blank serum control group (P < 0.01). ). Conclusion : Yiqi Tongluodan containing serum can arrest cells in G0G1 phase, inhibit cells from entering S phase, and have anti-VSMC proliferation effect, which may be one of the mechanisms of anti-coronary heart disease and cerebral thrombosis.