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目的研究碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对体外培养人牙髓细胞增殖、迁移活性的影响。方法以常规组织块培养法体外获得人牙髓细胞,实验组分别加入终浓度为1.0、5.0、10.0和50.0ng/ml的bFGF,对照组仅加入等量的空白培养基,采用CCK-8法分别测定450nm下各组光吸收度A值,研究bFGF对牙髓细胞增殖活性的影响;应用Transwell小室培养法,研究bFGF对牙髓细胞迁移活性的影响。结果与对照组相比,bFGF在1.0～50.0ng/ml浓度下可促进牙髓细胞的增殖(P<0.05),bFGF最低显效浓度为1.0ng/ml,最佳显效浓度为10.0ng/ml。bFGF在1.0～50.0ng/ml浓度下诱导细胞迁移(P<0.05)。结论 bFGF可诱导体外培养人牙髓细胞的增殖和迁移,在牙髓牙本质复合体修复中可能发挥重要作用。
Objective To investigate the effect of basic fibroblast growth factor (bFGF) on the proliferation and migration of human dental pulp cells in vitro. Methods Human dental pulp cells were obtained in vitro by conventional tissue culture method. The experimental groups were given bFGF at final concentrations of 1.0, 5.0, 10.0 and 50.0ng / ml respectively. The control group received only equal amount of blank medium and CCK-8 method The absorbance A of each group was measured at 450nm to study the effect of bFGF on the proliferation activity of dental pulp cells. The transwell chamber culture was used to study the effect of bFGF on the migration of dental pulp cells. Results Compared with the control group, bFGF promoted the proliferation of dental pulp cells at a concentration of 1.0-50.0ng / ml (P <0.05). The minimum effective concentration of bFGF was 1.0ng / ml and the optimal effective concentration was 10.0ng / ml. bFGF induced cell migration at concentrations of 1.0-50.0 ng / ml (P <0.05). Conclusion bFGF can induce the proliferation and migration of human dental pulp cells in vitro and may play an important role in the restoration of dental pulp dentin complex.