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目的 明确人鼻粘膜上皮细胞Na+通道的特性 ,为研究Na+通道在鼻粘膜病理性改变及治疗中的作用奠定理论基础。方法 利用膜片钳技术对经无血清气 液界面培养的鼻源性鼾症患者手术切除下鼻甲标本的鼻上皮细胞进行Na+通道基本特性研究。结果 在细胞贴附式膜片上 ,可记录到典型的单通道电流 ,其电导为 2 1 0 9pS ,反转电位为 - 50 96mV ,且 77 78%反转电位 <- 4 0mV ,离子通透性比率PNa/PK>5 80。在Na+通道抑制剂 10 4 mmol/LAmiloride存在于电极液内时 ,Na+通道发生率从 2 6 7%减少到 5 13% (P <0 0 5) ,表明大部分Na+通道可被Amiloride抑制。 10 3mmol/LCa2 +存在于浴液时 ,Na+通道的发生率无明显增加 (P >0 0 5)。电压对开放概率无明显影响。结论 在细胞贴附式膜片上 ,人鼻粘膜上皮细胞具有大部分Amiloride敏感型的Na+选择性通道及小部分Amiloride非敏感型的Na+通道。细胞外液的Ca2 +不能激活Na+选择性通道的开放 ,通道的开放概率是非电压依赖性的
Objective To clarify the characteristics of Na + channels in human nasal mucosa epithelial cells and lay a theoretical foundation for studying the role of Na + channels in the pathological changes and treatment of nasal mucosa. Methods The basic characteristics of Na + channel in nasal epithelial cells of inferior turbinate specimens were surgically excised from patients with nasal snoring cultured in serum-free gas-liquid interface by patch-clamp technique. Results A typical single channel current was recorded on a cell attachment membrane with a conductivity of 2 1 0 9 pS, an inversion potential of - 50 96 mV and a 77 78% inversion potential of <-4 0 mV with ion permeation Sex ratio PNa / PK> 5 80. When Na + channel inhibitor 10 4 mmol / LAmiloride was present in the electrode solution, the Na + channel rate decreased from 26.7% to 51.3% (P <0.05), indicating that most of the Na + channels were inhibited by Amiloride. There was no significant increase of Na + channel in the presence of 3 mmol / L Ca2 + in the bath (P> 0.05). Voltage has no significant effect on the open probability. Conclusions Human nasal mucosal epithelial cells have most of Amiloride-sensitive Na + selective channels and a small proportion of Amiloride non-sensitive Na + channels on cell attachment membranes. Ca2 + in extracellular fluid can not activate the opening of Na + selective channel. The open probability of channel is non-voltage-dependent