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目的:观察不同剂量的2-(2-苯并呋喃基)-2-咪唑啉[2-(2-benzofu-ranyl)-2-im idazoline,2-BFI]对脑缺血的神经保护作用以及对Bcl-2表达的影响。方法:采用线栓法建立大脑中动脉阻塞(MCAO)大鼠脑缺血模型,随机分为假手术组、模型组和2-BFI低、中、高剂量(1.5,3,6 mg.kg-1)组,通过TTC染色、HE染色、电镜、TUNEL染色、免疫组化等方法比较不同剂量的2-BFI对神经功能、梗死体积、脑组织病理、神经元超微结构、凋亡细胞及Bcl-2蛋白表达的影响。结果:2-BFI中剂量能显著减轻神经功能缺损、减小脑梗死体积、减轻脑组织病理损伤和神经元超微结构损伤、减少缺血半暗带凋亡细胞数、上调Bcl-2蛋白的表达,与模型组、2-BFI低剂量组、2-BFI高剂量组比较均有显著性差异。结论:中剂量(3 mg.kg-1)2-BFI对MCAO大鼠的神经保护作用最佳,能显著上调Bcl-2的表达。
OBJECTIVE: To observe the neuroprotective effects of 2- (2-benzofu-ranyl) -2-im idazoline (2-BFI) with different doses on cerebral ischemia and On Bcl-2 expression. Methods: The cerebral ischemia model of middle cerebral artery occlusion (MCAO) rats was established by thread occlusion and randomly divided into sham operation group, model group and low, medium and high dose of 2-BFI (1.5,3,6 mg.kg- 1) group. The effects of different doses of 2-BFI on nerve function, infarct volume, brain histopathology, neuronal ultrastructure, apoptotic cells and Bcl-2 were compared by TTC staining, HE staining, electron microscopy, TUNEL staining and immunohistochemistry. -2 protein expression. Results: The medium dose of 2-BFI could significantly reduce the neurological deficit, reduce the volume of cerebral infarction, alleviate the pathological damage of brain tissue and ultrastructure of neurons, reduce the number of apoptotic cells in ischemic penumbra, up-regulate Bcl-2 protein Expression, and the model group, 2-BFI low-dose group, 2-BFI high dose group were significantly different. CONCLUSION: Middle-dose (3 mg.kg-1) 2-BFI has the best neuroprotective effect on MCAO rats and significantly up-regulates Bcl-2 expression.