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以沙柳休眠芽茎段为试验材料,开展了沙柳休眠芽及嫩枝离体组织培养技术研究。研究结果表明诱导沙柳休眠芽萌生的最佳基本培养基为WPM培养基,叶片较绿,根较长,生长较快;诱导沙柳嫩枝生长的最适培养基激素配比为WPM+6-BA0.1mg·L~(-1)+NAA2mg·L~(-1),嫩枝较壮,高3.4 cm;诱导沙柳嫩枝生根的最适培养基为WPM+NAA0.2 mg·L~(-1),生根率达100%,6-BA会显著抑制沙柳生根。本研究为沙柳优良无性系的组培快繁、沙柳再生体系的建立以及利用基因工程手段改良沙柳遗传特性奠定了研究基础。
Salix psammophila buds were used as experimental material to study the dormancy buds of Salix psammophila and shoot tissue culture in vitro. The results showed that the best medium for inducing the dormancy buds of Salix psammophila was WPM medium, the leaves were green, the roots were longer and the growth was faster; the optimum medium for inducing the shoot growth of Salix psammophila was WPM + 6 -BA0.1mg · L -1 and NAA2mg · L -1, the shoots were stronger and 3.4 cm higher. The optimum culture medium for inducing the rooting of Salix psammophila was WPM + NAA0.2 mg · L ~ (-1) rooting rate of 100%, 6-BA will significantly inhibit Salix root. This study laid the foundation for the tissue culture and rapid propagation of Salix psammophila superior clones, the establishment of Salix psammophila regeneration system and the improvement of the genetic characteristics of Salix psammophila by genetic engineering.