论文部分内容阅读
目的:观察活血补肾方及其拆方对退变椎间盘组织IL-1β、TNF-α mRNA表达的影响。方法:采用纤维环针刺法复制腰椎间盘退变动物模型。造模成功后,将50只新西兰大白兔随机分为活血补肾方、活血方、补肾方、吲哚美辛及模型对照组5组,给药6周。采用逆转录聚合酶链反应检测IL-1β和TNFαmRNA表达情况。结果:与模型组比较,活血补肾方、补肾方、活血方和吲哚美辛组IL-1β、TNFα mRNA的表达均下调(P<0.01)。结论:活血补肾方具有抑制IL-1β、TNFα mRNA表达的作用,这可能是活血补肾方减轻退变椎间盘炎症反应,减缓椎间盘退变,治疗椎间盘源性腰痛的机理之一。
Objective: To observe the effect of Huoxue Bushen Recipe and its decomposed formulas on the expression of IL-1β and TNF-α mRNA in degenerative intervertebral disc. Methods: The animal model of lumbar intervertebral disc degeneration was replicated by fiber ring needle puncture. Fifty New Zealand white rabbits were randomly divided into five groups: Huoxue Bushen Fang, Huoxue Fang, Bushen Fang, Indomethacin and model control group for 6 weeks. Reverse transcription-polymerase chain reaction was used to detect the expression of IL-1β and TNFα mRNA. Results: Compared with the model group, the expressions of IL-1β and TNFα mRNA in Huoxue Bushen Recipe, Bushen Recipe, Huoxue Recipe and Indomethacin group were both decreased (P <0.01). Conclusion: Huoxue Bushen Recipe can inhibit the expression of IL-1β and TNFα mRNA, which may be one of the mechanisms of Huoxue Bushen Recipe in relieving the inflammatory reaction of degenerative disc and slowing the degeneration of intervertebral disc.