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ER-α36是一种新型雌激素受体亚型,广泛分布于乳腺、子宫、消化道、呼吸道等组织和细胞中。本文旨在研究ER-α36在中枢神经系统中的表达和分布情况。采用免疫细胞/组织化学方法和蛋白质免疫印迹杂交比较ER-α36在新生(1日龄)与成年(12周龄)Sprague-Dawley(SD)大鼠海马及皮层区的表达和分布情况。研究显示,ER-α36在成年大鼠海马及皮层区均有表达,主要分布于锥体神经元。ER-α36在新生大鼠海马和皮层神经元中均定位于细胞膜。新生大鼠皮层神经元ER-α36蛋白表达高于海马,而成年大鼠皮层神经元ER-α36蛋白表达低于海马。与新生大鼠相比较,成年大鼠海马和皮层神经元ER-α36表达均显著提高。以上结果提示,ER-α36参与介导神经元膜雌激素信号通路的调节,且在大鼠出生后海马及皮层发育中发挥着重要作用。因此,ER-α36可能为学习记忆、神经系统退行性疾病等的防治提供潜在的药物靶点。
ER-α36 is a new type of estrogen receptor, widely distributed in breast, uterus, digestive tract, respiratory and other tissues and cells. This article aims to study the expression and distribution of ER-α36 in the central nervous system. The expression and distribution of ER-α36 in the hippocampus and cortex of newborn (1 day old) and adult (12 weeks old) Sprague-Dawley (SD) rats were compared by immunocytochemistry / histochemistry and Western blotting. Studies have shown that, ER-α36 expression in hippocampus and cortex areas of adult rats, mainly in pyramidal neurons. ER-α36 localized in the plasma membrane in neonatal rat hippocampus and cortical neurons. The expression of ER-α36 protein in neonatal rat cortical neurons was higher than that in the hippocampus, while the expression of ER-α36 protein in adult rat cortical neurons was lower than that in the hippocampus. Compared with neonatal rats, ER-α36 expression in hippocampus and cortex neurons of adult rats were significantly increased. The above results suggest that ER-α36 is involved in the regulation of neuronal membrane estrogen signaling pathway and plays an important role in hippocampal and cortical development after birth in rats. Therefore, ER-α36 may provide potential drug targets for the prevention and treatment of learning and memory, neurodegenerative diseases and the like.