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目的建立同时测定甲嘎松汤中两种成分含量的高效液相色谱法,采用正交设计法优选甲嘎松汤中有效成分的提取工艺。方法以6-姜辣素和去氢二异丁香酚的总含量为指标,采用正交实验,对提取时间、乙醇体积分数及乙醇用量进行考察,优化提取条件。结果最佳提取工艺条件为加30倍90%的乙醇,超声30 min。结论该提取工艺合理,有效成分提取效率较高。
OBJECTIVE To establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of two components in Jiaga Song Decoction. The orthogonal design method was used to optimize the extraction of the active ingredients from Jiaga Song Decoction. Methods Taking the total content of 6-gingerol and dehydro-eugenol as indexes, the orthogonal experiment was used to study the extraction time, the volume fraction of ethanol and the amount of ethanol, and optimize the extraction conditions. Results The best extraction conditions were 30 times 90% ethanol, ultrasound 30 min. Conclusion The extraction process is reasonable and the extraction efficiency of active ingredients is high.