论文部分内容阅读
[目的]探索应用人类肝细胞肝癌细胞株建立裸鼠皮下-肝脏原位肝癌模型,及抑癌基因PTEN在该模型中的表达。[方法]应用Bel-7402肝癌细胞株建立裸小鼠皮下-肝脏原位肝癌模型48只,12只正常裸小鼠作对照;采用二步法免疫组化方法检测PTEN的表达。[结果]4周后解剖结果证实成模率100%;与正常组对比,模型组动物生存时间明显缩短,肝脏和脾脏明显增大(P<0.05或<0.01)。瘤体积与模型生存时间负相关,与脾指数正相关(P<0.01)。模型表现出明显的肝脏炎症反应和瘀胆。肝内肿瘤转移率80.0%,肺内肿瘤转移率10.4%。PTEN基因表达强度:癌旁组织>正常肝组织>癌组织(P<0.01)。[结论]该模型用于研究人类原发性肝癌,造模时间短、成瘤率高,生物学行为与人类肝癌接近,肿瘤体积过大是瘤鼠致死的重要因素。
[Objective] To explore the application of human hepatocellular carcinoma cell line to establish a model of orthotopic liver cancer in nude mice and the expression of tumor suppressor gene PTEN in this model. [Method] Forty-eight nude mice model of orthotopic liver cancer and 12 normal nude mice were established by Bel-7402 hepatoma cell line. Two-step immunohistochemical method was used to detect the expression of PTEN. [Results] After 4 weeks, the anatomical results confirmed that the rate of model formation was 100%. Compared with the normal group, the survival time of model group was significantly shortened and the liver and spleen were significantly increased (P <0.05 or <0.01). Tumor volume was negatively correlated with model survival time and positively correlated with spleen index (P <0.01). The model showed significant liver inflammation and stasis. Intrahepatic tumor metastasis rate of 80.0%, lung tumor metastasis rate of 10.4%. PTEN gene expression intensity: paracancer tissue> normal liver tissue> cancer tissue (P <0.01). [Conclusion] This model is mainly used to study human primary hepatocellular carcinoma (HCC), its short time of modeling, high rate of tumorigenesis, close biological behavior with human liver cancer and excessive tumor size.