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目的调查庚型肝炎病毒（ＨＧＶ）在静脉毒瘾者中的感染状况。方法采用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）检测８４例静脉毒瘾者血浆标本。ＨＧＶＲＮＡ经热变性法提取后逆转录为ｃＤＮＡ，在ＨＧＶ５′非编码区（５′ＮＣＲ）设计两对引物进行巢式扩增，产物为２３８ｂｐ，并经限制性内切酶ＨｐａⅡ鉴定扩增产物来自ＨＧＶ。结果８４例中有１５例为ＨＧＶＲＮＡ阳性，阳性率为１７．９％。ＨＧＶＲＮＡ阳性病例中１１例合并丙型肝炎病毒感染（１１／１５）。结论静脉毒瘾者是ＨＧＶ感染的高危人群；不洁注射是获得ＨＧＶ感染的重要途径。
Objective To investigate the infection status of Hepatitis G virus (HGV) in intravenous drug addicts. Methods 84 plasma samples from intravenous drug users were detected by reverse transcription-polymerase chain reaction (RT-PCR). HGVRNA was reverse transcribed into cDNA by heat denaturation and two pairs of primers were designed for nested amplification in the non-coding region of HGV 5 ’(5’NCR). The product was 238 bp in length and identified by restriction endonuclease HpaII. The amplified product was obtained from HGV. Results Of 84 cases, 15 cases were positive for HGVRNA, the positive rate was 17.9%. Eleven of the HGV RNA positive cases had hepatitis C virus infection (11/15). Conclusions Intravenous drug addicts are at high risk of HGV infection; unclear injection is an important way to obtain HGV infection.