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为研究中国汉族缺血性脑血管病(ICVD)患者 ApoE 基因型及等位基因频率分布状况,随机选择 ICVD 患者93例,平均年龄63.9岁;并选择同龄非 ICVD 受检者100例作为对照。采用单链构象多态性(SSCP)技术检测 ApoE 基因型。主要结果如下:(1)ICVD 患者组 ApoEε_(3/3)频率明显低于同龄对照组(P<0.05),(2)中年组 ICVD 患者中ε_(3/3)为56.78%,明显低于同龄对照组之73.07%(P<0.05),ε_(4/3)频率(31.37%)较同龄对照组(11.54%)增高(P<0.05).ε_4等位基因频率(19.61%)显著高于同龄对照组(9.62%)(P<0.05)。本实验结果提示:ApoEε_4基因频率增高与ICVD 发病有关,似可作为 ICVD 的危险标志,但其与老年患者关系不明显。
To study the distribution of ApoE genotype and allele frequencies in Chinese Han patients with ischemic cerebrovascular disease (ICVD), 93 ICCD patients were randomly selected, with an average age of 63.9 years. 100 non-ICVD subjects of the same age were selected as controls. ApoE genotypes were detected by single strand conformation polymorphism (SSCP). The main results are as follows: (1) The frequency of ApoE_ 3/3 in ICVD patients was significantly lower than that in the same age group (P <0.05). (2) The ε_ (3/3) in ICVD patients was 56.78% The frequency of ε_ (4/3) (31.37%) was higher than that of the control group (11.54%) (P <0.05), and the frequency of ε_4 allele (19.61%) was significantly higher than that of the control group (73.07% In the same age control group (9.62%) (P <0.05). The results suggest that: ApoEε_4 gene frequency increased with the incidence of ICVD, may be used as a risk marker for ICVD, but its relationship with elderly patients is not obvious.