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研究了二甲酚橙(XO)在乙醇-无机盐双水相萃取体系中的萃取行为,对无机盐种类、缓冲溶液和其用量等条件进行了优化,在最优条件下研究了上下层体积与萃取率、分配比等因素的变化规律。结果表明:在使用K2HPO4-KH2PO4缓冲溶液5.0 mL,95%乙醇6.0 mL,K2HPO4 6.0g,乙醇和蒸馏水体积比为1:2的条件下,获得了最大萃取率96.5%,回归方程y=0.0004+0.0263x,相关系数r=0.9995,摩尔吸光系数ε=1.9×104 L/(mol.cm),线性范围0～25μg/mL。回收率为96.3%～98.5%。
The extraction behavior of xylenol orange (XO) in ethanol-inorganic acid aqueous two-phase extraction system was studied. The conditions of inorganic salts, buffer solution and their dosage were optimized. Under the optimal conditions, And extraction rate, distribution ratio and other factors change. The results showed that the maximum extraction yield was 96.5% under the conditions of 5.0 mL of K2HPO4-KH2PO4 buffer, 6.0 mL of 95% ethanol, 6.0 g of K2HPO4 and 1: 2 of ethanol and distilled water. The regression equation y = 0.0004 + 0.0263x, the correlation coefficient r = 0.9995, the molar absorptivity ε = 1.9 × 104 L / (mol.cm), the linear range 0 ~ 25μg / mL. The recovery rate was 96.3% ~ 98.5%.