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目的研究一种从鲨鱼软骨(shark cartilag,SC)中简便、快速提取低分子蛋白的方法,并证实该方法提取的鲨鱼软骨低分子蛋白能有效地抑制新生血管的形成。方法以鲨鱼软骨为原料,粉碎后反复冻融,离心去沉淀,超滤、真空干燥后得到鲨鱼软骨低分子蛋白。体外实验测定它对内皮细胞增殖、黏附和迁移的影响,用鸡胚绒毛尿囊膜实验测定对血管生成的影响。结果鲨鱼软骨低分子蛋白能显著抑制血管内皮细胞的生长[MTT法抑制率为(42.026±3.530%)]、黏附[抑制率为(66.783±5.220)%]和迁移运动[抑制率为(86.760±12.487)%],可显著抑制鸡胚绒毛尿囊膜的新生血管的形成。结论鲨鱼软骨低分子蛋白可以抑制内皮细胞生长、黏附和迁移运动,进而抑制新生血管的生长。
Objective To study a simple and rapid method for extracting low molecular weight proteins from shark cartilage (SC), and to confirm that shark cartilage low molecular weight protein extracted from this method can effectively inhibit angiogenesis. Methods shark cartilage as raw material, after crushing repeated freezing and thawing, centrifugation to precipitation, ultrafiltration, vacuum drying shark cartilage low molecular weight protein. In vitro experiments to determine its impact on endothelial cell proliferation, adhesion and migration, with chick chorioallantoic membrane assay on angiogenesis. Results The shark cartilage low molecular weight protein could significantly inhibit the growth of vascular endothelial cells [MTT assay (42.026 ± 3.530%], adhesion rate (66.783 ± 5.220)%] and migration assay (inhibition rate: 86.760 ± 12.487)%], can significantly inhibit chick chorioallantoic membrane formation of neovascularization. Conclusion Shark cartilage low molecular weight protein can inhibit the growth, adhesion and migration of endothelial cells and thus inhibit the growth of neovascularization.