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深低温保存淋巴细胞在移植免疫学、免疫试验质控和治疗,以及老年医学研究中有重要应用价值。采用程序降温仪实施自动程序降温是国外目前研究最多,并且是迄今最有效的冻存淋巴细胞的方法。我们利用国产装置建立了自动程序降温冻存小鼠脾淋巴细胞的方法,取得良好的效果,现将实验结果报告如下。
Cryopreservation of lymphocytes in transplantation immunology, quality control and treatment of immune tests, as well as geriatric research has important application value. The use of program cooling device to implement automatic program cooling is currently the most studied, and is by far the most effective method of cryopreserved lymphocytes. We use a homemade device to establish a method of automatic procedure to cool the spleen lymphocytes of mice and achieve good results. The experimental results are reported as follows.