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本文利用肝脏羟化酶及肾脏羟化酶,对维生素D_3的体外25位羟化作用及1α位羟化作用进行了初步研究。结果表明:应用佝偻病鸡的肝线粒体及微粒体悬液,分别与维生素D_3、NADPH孵化系统在体外进行孵育,均可得到羟化产物—25-OH-D_3,线粒体维生素D_3-25羟化酶的活性为0.49Pmol 25-OH-D_3/2h·10mg pr,微粒体为0.28 pmol 25-OH-D_3/2h·10mg pr。二者转化率分别为11.9％与5.8％,线粒体25-羟化酶活性明显高于微粒体。应用佝偻病鸡的肾线粒体悬液,与25-OH-D_3、NADPH孵化系统在体外进行孵育,可以获得羟化产物-1a,25-(OH)_2-D_3,其转化率为37.5％。
In this paper, liver hydroxylase and renal hydroxylase, vitamin D_3 in vitro 25 hydroxylation and 1α hydroxylation were studied. The results showed that both the mitochondrial and microsomal suspensions of rickets chicken were incubated in vitro with vitamin D 3 and NADPH incubator system respectively. The hydroxylation products of -25-OH-D 3 and mitochondrial vitamin D 3-25 hydroxylase The activity was 0.49 Pmol 25-OH-D 3 / 2h · 10 mg pr and the microsome was 0.28 pmol 25-OH-D 3 / 2h · 10 mg pr. The conversion rates of the two were 11.9% and 5.8%, respectively. The mitochondrial 25-hydroxylase activity was significantly higher than that of the microsome. The rhesus kidney chicken mitochondrial suspension was incubated with 25-OH-D_3 and NADPH in vitro. The hydroxylation product-1a, 25- (OH) _2-D_3 was obtained and the conversion rate was 37.5%.