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目的:采用UPLC测定11批全缘叶绿绒蒿花中槲皮素-3-O-β-D-葡萄糖-(1→6)-β-D-葡萄糖苷(M1),槲皮素-3-O-[2-O-乙酰基-β-D-葡萄糖-(1→6)-β-D-葡萄糖苷](M2)和异槲皮苷(M3)的含量。方法:采用超声提取法,提取溶剂为体积分数90%甲醇,提取时间为45 min,对全缘叶绿绒蒿样品进行处理。色谱柱为ACQUITY UPLC HSS T3(2.1 mm×100 mm,1.8μm),流速300μL·min~(-1),柱温35℃,检测波长255 nm,以乙腈-0.1%冰乙酸水(16∶84)为流动相,洗脱时间10 min。结果:在11批全缘叶绿绒蒿花样品中,不同产地、不同采集时间的样品所含有的3种黄酮类化学成分含量各不相同,但总体上两种槲皮素葡萄糖苷含量较高,而异槲皮苷的含量较低;马尔康县梦笔山所采样品黄酮苷总含量高于其他采集地,5月和6月采集的样品黄酮苷总含量高于7月。结论:全缘叶绿绒蒿花中所含主要化学成分含量随产地、采收月份不同而有所差异。该方法准确且具有较高灵敏度,可较准确反应全缘叶绿绒蒿花中化学成分的含量,可为全缘叶绿绒蒿花的质量评价和质量标准提高提供依据。
Objective: To determine the contents of quercetin -3-O-β-D-glucose- (1 → 6) -β-D-glucoside (M1), quercetin-3 -O- [2-O-acetyl-β-D-glucose- (1 → 6) -β-D-glucoside] (M2) and isoquercitrin (M3) Methods: Ultrasonic extraction was used to extract 90% methanol with a volume fraction of 45%. The extraction time was 45 min. The chromatographic column was ACQUITY UPLC HSS T3 (2.1 mm × 100 mm, 1.8 μm) with a flow rate of 300 μL · min -1. The column temperature was 35 ° C and the detection wavelength was set at 255 nm with acetonitrile-0.1% glacial acetic acid (16:84 ) As the mobile phase, elution time 10 min. Results: Among the 11 samples of Artemisia annua L., the contents of three flavonoids contained in the samples of different origins and different collection time were different, but the contents of two kinds of quercetin glucosides were generally high While the content of isoquercitrin was lower. The total content of flavonoid glycosides in Mabangshan Mountain was higher than that in other sites. The total flavonoid glycosides content in May and June was higher than that in July. Conclusion: The main chemical constituents contained in Artemisia annua L. vary with the place of production and the month of harvest. The method is accurate and has high sensitivity, which can accurately reflect the chemical constituents in Artemisia annua L., which can provide the basis for quality evaluation and quality standard improvement of Artemisia annua.