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目的 探讨不同剂量尤其是低剂量的γ干扰素(IFN γ)体内表达对小鼠H2 2肝癌发生发展的影响。方法 利用小鼠H2 2肝癌体内发生和进展的模型,以不同剂量IFN γ表达质粒(mIFNG)进行长期和短期直接肌肉转染,检测H2 2肝癌的发生率及生长速率;半定量RT PCR、ELISA及实时定量PCR检测IFN -γ的表达情况;肌肉转染表达IFN -γ阻断剂,检测其对IFN -γ作用的影响。结果 注射低剂量(10 μg)的mIFNG质粒局部持续表达IFN γ可明显促进小鼠H2 2肝癌的发生和发展,然而短暂的表达则没有这种促进作用。IFN -γ拮抗剂则可对抗低剂量表达IFN -γ对肿瘤的促进作用。另一方面,注射高剂量(10 0 μg)的mIFNG质粒局部持续表达IFN -γ则能够介导明显的抗肿瘤效应。结论IFN γ对小鼠H2 2肝癌具有双重作用,也可能是联系慢性炎症与肿瘤发生发展之间的一个十分重要的衔接者。
Objective To investigate the effects of different doses of interferon gamma (IFNγ) in vivo on the development of H 2 2 hepatocarcinoma in mice. Methods The mouse H 2 2 hepatocarcinoma was induced in vivo and in vivo by direct and long-term muscle transfection with different doses of IFNγ expression plasmid (mIFNG) to detect the incidence and growth rate of H 2 2 hepatocarcinoma. Semi-quantitative RT-PCR and ELISA And real-time quantitative PCR were used to detect the expression of IFN-γ. The muscle was transfected with IFN-γ blocker to detect the effect of IFN-γ. Results The sustained expression of IFNγ by low dose (10 μg) mIFNG plasmid could significantly promote the development and progression of H 2 2 hepatocarcinoma in mice, however, transient expression did not. IFN-γ antagonists can be against low-dose expression of IFN-γ on the promotion of cancer. On the other hand, injection of high dose (100 μg) of mIFNG plasmid locally and consistently expressing IFN-γ mediated a marked anti-tumor effect. Conclusions IFNγ has a dual effect on H2O2-induced HCC in mice and may be a very important link between chronic inflammation and tumorigenesis.