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目的:探讨基因联合放疗对小鼠B16移植肿瘤的抑制作用。方法:碱裂解法提取纯化质粒DNA,微量注射法直接将质粒DNA导入体内肿瘤;建立荷瘤小鼠模型,于小鼠右后肢皮下接种5×105个B16黑色素瘤细胞,待肿瘤直径达0.3~0.5 cm时开始治疗;肿瘤局部注射pNE-mIL-12和pcDNA-B7-1质粒3次,并给予3次X线照射,观察小鼠移植肿瘤的生长情况。结果:pNE-mIL-12重组质粒与pcDNA-B7-1质粒联合2 Gy放射治疗组肿瘤生长速率最低,小鼠生存时间明显延长(P<0.01~P<0.001),末次照射后31 d小鼠死亡率仅为22.2%,而且其中有1只小鼠肿瘤消退。结论:多基因联合放疗可有效抑制小鼠B16移植肿瘤的生长,其效果好于单基因治疗和单纯放疗。
Objective: To investigate the inhibitory effect of gene combined with radiotherapy on the B16 tumor in mice. Methods: Plasmid DNA was extracted by alkaline lysis method and plasmid DNA was directly introduced into tumor in vivo by microinjection method. A tumor-bearing mouse model was established. 5 × 105 B16 melanoma cells were inoculated subcutaneously into the right hind limb of mice until the tumor diameter reached 0.3 ~ The tumors were treated with pNE-mIL-12 and pcDNA-B7-1 plasmids three times and three times X-ray irradiation. The growth of mice transplanted tumor was observed. Results: The tumor growth rate of pNE-mIL-12 recombinant plasmid combined with pcDNA-B7-1 plasmid was the lowest, the survival time of mice was significantly prolonged (P <0.01 ~ P <0.001) The death rate was only 22.2%, and one of the mice had a regression of the tumor. Conclusion: Multi-gene combined with radiotherapy can effectively inhibit the growth of mouse B16 tumor, which is better than single gene therapy and radiotherapy alone.