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用《消毒技术规范》的细菌培养方法和乙肝表面抗原测定法(ELISA、SPRIA).对医院常用10种消毒液共332份样品进行了污染调查,结果为:细菌总超标率为11.7%.HBsAg总检出率为5.83%;细菌污染以0.1%洗消粉最严重(超标率50%).其次为75%乙醇(11.7%),1%碘伏(8.3%),2.5%碘酒(10.8%及器械液(4.7%);而消毒液HBsAg污染以75%乙醇为最高(12.9%),其次为器械液(8.1%),这与两种消毒剂不能杀灭乙肝病毒结果一致。戊二醛类消毒液如“一消净”等没有细菌和HBsAg污染。结果提示加强消毒产品管理,使用高效稳定的消毒液代替低质的消毒剂,对于降低消毒液污染,减少侵入性器械带菌、控制院内感染具有重要意义。
Bacterial culture methods and the hepatitis B surface antigen assay (ELISA, SPRIA) using the “Sterilization Specification”. A total of 332 samples of 10 kinds of disinfectant commonly used in hospitals were investigated for contamination. The results showed that the total bacterial over-standard rate was 11.7%. The total detection rate of HBsAg was 5.83%; bacterial contamination was most serious with 0.1% detergent powder (exceeding the standard rate of 50%). Followed by 75% ethanol (11.7%), 1% iodophor (8.3%), 2.5% iodine (10.8% 75% ethanol is the highest (12.9%), followed by the instrument solution (8.1%), which is consistent with the results of two kinds of disinfectants can not kill hepatitis B. The glutaraldehyde disinfectant such as “a clear net” and so on No bacteria and HBsAg contamination.Results suggest that strengthening the management of disinfection products, the use of highly efficient and stable disinfectant instead of low-quality disinfectant, disinfectant to reduce pollution, reduce invasive equipment, infection, control of nosocomial infection is of great significance.