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目的:检测人类染色体端粒酶基因在非典型增生向宫颈癌转变的过程中是否扩增。方法:118例患者宫颈液基细胞,其中CIN-Ⅰ组21例,CIN-Ⅱ组11例,CIN-Ⅲ组18例及宫颈癌患者(包括原位癌)38例,其中鳞癌29例,腺癌9例;宫颈炎患者30例为对照组,应用FISH技术检测宫颈液基细胞中人类染色体端粒酶基因扩增阳性率,并进行比较。结果:宫颈炎组扩增阳性率为10.00%;CIN组阳性率共为87.50%。其中CIN-Ⅰ组为85.71%,CIN-Ⅱ组为90.90%,CIN-Ⅲ为88.89%;宫颈癌组阳性率为89.47%;CIN组及宫颈癌组人类染色体端粒酶基因扩增信号均较对照组明显增多(P<0.01);宫颈癌组与CIN组比较,差异无统计学意义(P>0.05);鳞癌与腺癌比较差异有统计学意义(P<0.01)。结论:人类染色体端粒酶基因在非典型增生异常向宫颈癌转变的过程中均伴有该基因的扩增,且在宫颈癌中扩增更明显,宫颈癌的发生发展与人类染色体端粒酶基因扩增有联系。应用FISH技术检测人类染色体端粒酶基因有望成为子宫颈癌早期筛查方法之一。
OBJECTIVE: To detect whether the human telomerase gene is amplified in the process of atypical hyperplasia to cervical cancer. Methods: 118 cases of cervical liquid-based cells, CIN-Ⅰ group of 21 cases, CIN-Ⅱ group of 11 cases, CIN-Ⅲ group of 18 cases and cervical cancer patients (including carcinoma in situ) 38 cases, including squamous cell carcinoma in 29 cases, 9 cases of adenocarcinoma; 30 cases of cervicitis patients as control group, the positive rate of human chromosome telomerase gene amplification in cervical liquid-based cells was detected by FISH and compared. Results: The positive rate of cervicitis was 10.00%. The positive rate of CIN was 87.50%. The CIN-Ⅰ group was 85.71%, CIN-Ⅱ group was 90.90%, CIN-Ⅲ was 88.89%; The positive rate of cervical cancer group was 89.47%; The signal of telomerase amplification signal of human chromosome in CIN group and cervical cancer group were more (P <0.01). There was no significant difference between cervical cancer group and CIN group (P> 0.05). There was significant difference between squamous cell carcinoma and adenocarcinoma (P <0.01). CONCLUSION: Human chromosome telomerase gene is amplified during the process of atypical hyperplasia to cervical cancer, and its amplification is more obvious in cervical cancer. The occurrence and development of cervical cancer is related to human chromosome telomerase Gene amplification is linked. Application of FISH technology to detect human chromosome telomerase gene is expected to be one of the early screening methods for cervical cancer.