诊断布病常用的血清学方法,在检验野毒布氏菌感染人或动物的价值和意义的材料过去有些报导。但是,这些方法对免疫动物,特别是应用M_5号菌苗连续免疫的牛,它们出现的结果如何,见到的报告不多。因此,我们于1987年对M_5号菌苗不同免疫次数的1010头成牛,应用五种血清学方法检查。现将实验观察结果报告如下。 Some commonly used serological methods for the diagnosis of brucellosis have been reported in the past to examine the value and significance of M. bovis infection in humans or animals. However, the results of these methods on immunized animals, especially those vaccinated continuously using M_5 vaccine, are not well documented. Therefore, in 1987, we made 1010 calves of different immunizations against M_5 vaccine using five serological methods. The experimental observations are reported below.