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目的对RhD弱阳性儿童患者进行RhD变异体检测分析。方法用微柱凝胶血型鉴定卡及盐水试管法进行RhD血型初筛;微柱凝胶抗球法进行弱D确认试验;确认试验RhD弱阳性者进行PCR-SSP及RHD外显子测序分析。结果 RhD初筛阴性46例(0.38%),确认实验检出弱阳性4例(8.7%);4例RhD弱阳性标本经基因检测,1例1227A杂合子(Del型),1例DⅢa型,2例弱D15型。结论 Del型也可能被血清学方法检出;RhD弱阳性患者中抗原数量减少者较多;针对RhD阳性红细胞是否产生需进一步研究。
Objective To detect and analyze RhD variant in children with weak RhD positive. Methods RhD blood type was preliminarily screened by micro-column gel blood type identification card and saline test tube method. The micro-column gel anti-ball method was used to confirm the weak D test. PCR-SSP and RHD exon sequencing analysis were confirmed in patients with weak RhD. Results The negative results of RhD screening in 46 cases (0.38%) were confirmed weak positive in 4 cases (8.7%). Four cases of RhD weak positive samples were detected by genetic testing, 1 case of 1227A heterozygote (Del), 1 case of DⅢa, 2 cases of weak D15 type. Conclusion Del type may also be detected by serological methods; RhD weak positive patients reduce the number of more antigens; for RhD-positive red blood cells produce further research.