Assessing the fatigue life of mooring systems is important for deep water structures. In this paper, a comprehensive fatigue analysis is conducted on the mooring lines applied in a semi-submersible platform with special focus on the low frequency(LF) fatigue damage. Several influential factors, including water depth, wave spectral parameters, and riser system, are considered. Numerical simulation of a semi-submersible platform with the mooring/riser system is executed under different conditions, and the fatigue damage of mooring lines is assessed by using the time domain analysis method as a benchmark. The effects of these factors on the mooring line tension and the fatigue damage are investigated and discussed in detail. Research results indicate that the LF fatigue damage only accounts for a very small portion of the total damage, although the LF components dominate the global motion response and the mooring line tension of the semi-submersible platform. However, it is demonstrated that the LF fatigue damage is clearly affected by the influential factors. The increase in water depth and spectral peak periods, and the existence of risers can weaken the contribution of the LF components to the mooring line fatigue damage, while the fatigue damage due to the LF components increases with the increase of significant wave height. Assessing the fatigue life of mooring systems is important for deep water structures. In this paper, a comprehensive fatigue analysis is conducted on the mooring lines applied in a semi-submersible platform with special focus on the low frequency (LF) fatigue damage. Factors, including water depth, wave spectral parameters, and riser system, are considered. by using the time of the mooring / riser system is executed under different conditions, and the fatigue damage of mooring lines is using by the time The effects of these factors on the mooring line tension and the fatigue damage are investigated and discussed in detail. Research results indicate that the LF fatigue damage only accounts for a very small portion of the total damage, although the LF components dominate the global motion response and the mooring line tension of the semi-submersible platform. However, it is demonstrated t The increase in water depth and spectral peak periods, and the existence of risers can weaken the contribution of the LF components to the mooring line fatigue damage, while the fatigue damage due to the LF components increases with the increase of significant wave height.