目的 :探讨 Ad CMVtk/GCV系统诱导肿瘤细胞死亡的途径。方法 :采用台盼蓝染色检测坏死细胞数 ,吖啶橙荧光染色检测凋亡细胞数 ,同时观察凋亡细胞的形态学改变。结果 :Ad CMVtk/GCV系统诱导肺癌 ( Lewis)细胞死亡形式有两种 ,即坏死和凋亡 ,先出现凋亡 ,后引起坏死。结论 :HE染色及吖啶橙荧光染色可见典型的凋亡形态学改变。 Objective: To explore the way of AdCMVtk / GCV induced tumor cell death. Methods: The number of necrotic cells was detected by trypan blue staining, the number of apoptotic cells was detected by acridine orange fluorescence staining, and the morphological changes of apoptotic cells were observed. RESULTS: AdCMVtk / GCV induced Lewis lung cell death in two forms: necrosis and apoptosis, with apoptosis first and necrosis afterwards. Conclusion: Typical apoptotic morphological changes can be observed by HE staining and acridine orange fluorescence staining.