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目的探索用逆转录病毒作载体介导HyTK基因转移入人膀胱癌细胞中表达以及HyTK基因转移联合丙氧鸟苷(GCV)对体外人膀胱癌细胞的杀伤作用。方法含HyTK基因的逆转录病毒载体LHyTKN,经包装细胞PA317包装后,获得重组逆转录病毒;病毒感染人膀胱癌细胞株EJ并进行潮
Objective To explore the killing effect of HyTK gene transfer combined with ganciclovir (GCV) on the human bladder carcinoma cells in vitro using retroviral vector-mediated HyTK gene transfer into human bladder cancer cells. Methods The retroviral vector containing HyTK gene, LHyTKN, was packaged in packaging cells PA317 to obtain recombinant retrovirus. The virus was infected into human bladder cancer cell line EJ and subjected to tide