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生物分子的识别、检测技术是生物分子学和生物医学研究中极为重要的领域。光波导模式谱(opticalwaveguidelightmodespectroscopy,OWLS)是一种非标记光学检测方法。OWLS方法通过检测波导附近的有效折射率的变化进行传感,无需进行放射性、荧光、或其他标记方法。通过同时对TM/TE两种传导模式的测定,可以实时定量分析波导表面吸附物质的质量。OWLS目前已经被应用到生物大分子相互作用、生物材料表征、环境及食品安全监测等领域。总结了以OWLS为原理构建的光栅生物传感器(opticalgratingcouplerbiosensor,OGCB)的原理,与其他非标记方法在传感指标方面的比较及应用领域的最新国外研究进展,并对该传感器的发展方向进行预测。
Identification and detection of biomolecules are extremely important fields in biomolecular and biomedical research. Opticalwaveguidelightmodespectroscopy (OWLS) is a non-labeled optical detection method. The OWLS method senses the change in effective refractive index near the waveguide without the need for radioactive, fluorescent, or other labeling methods. Through the simultaneous determination of TM / TE conduction modes, the mass of adsorbed material on the surface of the waveguide can be quantitatively analyzed in real time. OWLS has been applied to such fields as biological macromolecule interaction, biological material characterization, environment and food safety monitoring. The principle of OGCB, the principle of OGCB, and the comparison with other non-marking methods in sensing indices and the latest research progress in the field of application are summarized. And the development direction of the sensor is predicted.