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通过Snef1216发酵液处理番茄根系和包衣种子,研究番茄抗南方根结线虫的组织病理学变化和作用方式。结果显示:处理番茄根系且接种15 d,应答根系内根结线虫的数量减少47.5%,且巨细胞出现空泡;包衣番茄种子,接种4、8和12 d,根系内2龄幼虫分别减少54.1%、3.4%和41.7%,接种10、20和30 d,根系内2龄、3龄、4龄幼虫和雌虫的数量也均比对照组番茄明显减少。表明Snef1216发酵液诱导了番茄对南方根结线虫的抗性。
The tomato roots and the coated seeds were treated with Snef1216 fermentation broth to study the histopathological changes and the mode of action of tomato against Meloidogyne incognita. The results showed that the number of root-knot nematodes in response roots was reduced by 47.5% and the giant cells were vacuolized after 15 days of inoculation. After the tomato seeds were coated for 4, 8 and 12 days, the number of 2nd instar larvae in the root system decreased 54.1%, 3.4% and 41.7% respectively. After inoculation for 10, 20 and 30 d, the number of 2nd, 3rd, 4th instar larvae and females in the root system also decreased significantly compared with that of the control group. This indicated that Snef1216 fermentation broth induced tomato resistance to Meloidogyne incognita.