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根据甘蓝测序结果,利用CodonW软件对结球甘蓝的48 000个蛋白质编码基因序列进行了分析,计算出同义密码子相对使用频率,确定了TCT、CCT、AGA、GTT、GCT、GAT等6个高频密码子。将甘蓝与大肠杆菌、拟南芥、棉花、水稻的密码子使用频率进行了比较,发现甘蓝密码子使用偏爱性与同为双子叶植物的拟南芥、棉花基本一致,而与大肠杆菌及单子叶植物水稻均具有较大差异。以甘蓝密码子用法分析结果为依据,对Bt cry1C抗虫基因进行了密码子的改造,得到了具有甘蓝密码子使用特点的cry1C基因序列,为甘蓝的转基因研究奠定了分子基础。
According to the sequencing results of cabbage, 48,000 protein coding genes of cabbage were analyzed by CodonW software, and the relative frequency of synonymous codons was calculated, including 6 TCT, CCT, AGA, GTT, GCT and GAT High frequency codon. The frequency of codon usage in cabbage and E. coli, Arabidopsis thaliana, cotton and rice was compared. It was found that the preference for codon usage in cabbage was the same as that of dicotyledonous Arabidopsis thaliana and cotton, Leaf plant rice are quite different. Based on the analysis of the codon usage of Brassica oleracea, the cry1C gene of Bt cry1C gene was modified and the cry1C gene sequence was obtained, which laid the molecular foundation for the transgenic study of cabbage.