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以叶片为外植体,对条叶唇柱苣苔(Chirita ophiopogoides)进行离体培养与快速繁殖研究。结果表明,培养基MS+0.1 mg/L 6-BA+0.1 mg/L NAA适用于初代培养中的芽诱导和植株再生;培养基MS+0.5 mg/L 6-BA+0.1mg/L NAA+10%香蕉+5%马铃薯和MS+0.5mg/L 6-BA+0.5 mg/L NAA+2%香蕉分别适用于继代增殖和壮苗培养,繁殖系数分别为7.9倍/60 d和5.6倍/60 d;培养基MS适用于生根培养,培养30 d后生根率达到100%;以腐质土为基质,将条叶唇柱苣苔生根试管苗移栽到大棚,成活率达92.5%。根据上述快繁技术,理论上每株试管苗每年可繁殖条叶唇柱苣苔种苗20 176株。
Leaf explants were used for in vitro culture and rapid propagation of Chirita ophiopogoides. The results showed that medium supplemented with MS + 0.1 mg / L 6-BA + 0.1 mg / L NAA was suitable for shoot induction and plant regeneration in primary culture. MS medium supplemented with 0.5 mg / L 6-BA + 0.1 mg / L NAA + 10% banana + 5% potato and MS + 0.5 mg / L 6-BA + 0.5 mg / L NAA + 2% banana were suitable for subculture and seedling culture respectively with multiplication coefficients of 7.9 times / 60 days and 5.6 times / 60 d. The medium MS was suitable for rooting culture, and the rooting rate reached 100% after 30 days of culture. Using humus soil as the substrate, the survival rate of 92.5% was transplanted into the greenhouse by using the culture tube MS. According to the above propagation technology, in theory, each plant test tube seedlings can breed Labiatae 20 20 seedlings.