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收集了我院1994年10月至1995年4月金黄色葡萄球菌100株,应用药敏试验法筛选 MR-SA,并检测其对12种抗生素的耐药性,计算 MIC_(50)和 MIC_(90)。应用乳胶凝集试验法,测定其肠毒素产生率。结果我院总的 MRSA 阳性率为60%,烧伤科的 MRSA 阳性率为67.4%,内科62.5%,骨科44.5%。MRSA 耐药性低于50%的有万古霉素(3.3%)、去甲万古霉素(5%)、丁胺卡那霉素(21.5%)3种。MR-SA 产肠毒素率为100%,其中产两种以上者占35%,而 MSSA 产肠毒素率仅为20%,其中产两种以上者占5%。
100 strains of Staphylococcus aureus were collected from October 1994 to April 1995 in our hospital. MR-SA was screened by drug susceptibility test and its resistance to 12 antibiotics was tested. The MIC50 and MIC_ (50) 90). The latex agglutination test method was used to determine the enterotoxin production rate. Results The total positive rate of MRSA in our hospital was 60%. The positive rate of MRSA in burns department was 67.4%, 62.5% in internal medicine and 44.5% in orthopedics. Vancomycin (3.3%), norvancomycin (5%) and amikacin (21.5%) were less than 50% MRSA. The production of enterotoxins by MR-SA was 100%, with 35% of those producing more than 2 strains of MRSA. The rate of enterotoxins produced by MSSA was only 20%, with 5% of those producing more than 2 strains.