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目的 研究患者颈部转移性淋巴结中是否存在ＥＢ病毒。方法 ①采用聚合酶链反应（ＰＣＲ） 技术检测５０ 例患者的颈部淋巴结穿刺液；②采用ＰＣＲ法检测１５ 例石蜡包埋鼻咽癌（ＮＰＣ） 组织；③对１２例ＥＢ病毒ＶＣＡＩｇＡ 抗体阳性患者血清进行ＥＢ 病毒基因检测。结果 ①３０ 例ＮＰＣ 患者中，２７ 例ＥＢＶＤＮＡ 阳性（ 阳性率９０ ％ ） ；７ 例头颈部其他肿瘤患者中１ 例阳性（１４－３ ％ ） ；８ 例其他部位肿瘤患者中１ 例阳性（１２－５ ％ ） ；５ 例鼻咽部炎症患者ＥＢＶＤＮＡ 检测均为阴性。经χ２ 检验，ＮＰＣ 组与其他肿瘤及鼻咽部炎症组相比，阳性率差异有非常显著意义（ Ｐ＜ ０－００５） 。②１５ 例石蜡包埋ＮＰＣ 组织，ＥＢＶ基因阳性者有１２ 例（ 阳性率８０ ％ ） ；１ 例鼻咽部慢性粘膜炎石蜡包埋组织末检测到ＥＢＶＤＮＡ。③３ 例临床诊断为ＮＰＣ 的血清中均检测到ＥＢＶＤＮＡ（２５ ％ ） 。结论 对于隐性鼻咽癌和原发灶不明的颈部转移癌中确认是否为鼻咽癌，ＥＢ 病毒基因检测是有价值的，ＰＣＲ 技术可以为ＮＰＣ 的诊断和鉴别诊断提供参考依据。
Objective To study the presence or absence of Epstein-Barr virus in patients with metastatic lymph nodes in the neck. Methods ① The cervical lymph node puncture fluid of 50 patients was detected by polymerase chain reaction (PCR); ② 15 paraffin-embedded nasopharyngeal carcinoma (NPC) tissues were detected by PCR; ③ 12 cases of EBV VCA IgA antibody Positive patient serum EB virus gene test. Results ① Of the 30 patients with NPC, 27 were EBVDNApositive (positive rate was 90%), 7 were positive in other head and neck cancer patients (14-3%), and 1 was positive in 8 patients with other tumor sites 12-5%); 5 cases of nasopharyngeal inflammation in patients with EBV-DNA test were negative. By χ2 test, NPC group compared with other tumors and nasopharyngeal inflammation group, the positive rate difference was very significant (P <0-005). ②15 cases of paraffin embedded NPC tissue, EBV gene positive in 12 cases (positive rate of 80%); 1 case of nasopharyngeal chronic mucositis paraffin embedded tissue detected EBV DNA. ③ 3 cases of clinical diagnosis of NPC serum were detected in EBV DNA (25%). Conclusion The detection of Epstein-Barr virus (EBV) gene is valuable for the detection of recessive nasopharyngeal carcinoma and cervical metastatic carcinoma of unknown primary tumor. PCR can provide a reference for the diagnosis and differential diagnosis of NPC.