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目的:建立同时测定神农香菊中绿原酸和木犀草素含量的方法。方法:采用RP-HPLC法,色谱柱为Kromasil-C_(18)(250mm×4.6mm,5μm),流动相为甲醇(A)-乙腈(B)-0.8%磷酸溶液(C),进行梯度洗脱,流速为0.5ml·min~(-1),柱温30℃,检测波长为328nm。结果:绿原酸和木犀草素分别在4.5～90.0μg·ml~(-1)(r=0.999 3)和10.5～210.0μg·ml~(-1)(r=0.999 6)浓度范围内均有良好的线性关系,平均加样回收率分别为98.72%,97.77%。结论:本方法简便,快速,准确,重复性好,可用于神农香菊药材的质量控制。
Objective: To establish a method for simultaneous determination of chlorogenic acid and luteolin in Shennong Xiangju. Methods: The RP-HPLC method was used. The chromatographic column was Kromasil-C18 (250mm×4.6mm, 5μm). The mobile phase consisted of methanol (A)-acetonitrile (B)-0.8% phosphoric acid solution (C). Gradient washing was performed. The flow rate was 0.5ml·min~(-1), the column temperature was 30°C, and the detection wavelength was 328nm. Results: Chlorogenic acid and luteolin were in the range of 4.5-90.0 μg·ml -1 (r=0.999 3) and 10.5-210.0 μg·ml -1 (r=0.999 6) respectively. There is a good linear relationship, with an average recovery of 98.72% and 97.77%, respectively. Conclusion: The method is simple, rapid, accurate and reproducible. It can be used for the quality control of Shennong Xiangju medicinal materials.