目的探讨薄荷中总黄酮的提取、鉴别及其对羟自由基的清除作用。方法采用超声波乙醇浸提法提取薄荷中的黄酮类物质,用分光光度法测定含量,并做黄酮类物质的羟自由基清除作用试验。结果测得样品中总黄酮的含量为0.325mg·mL-1,回收率为99.53%,其纯度和产率均较高。结论超声波乙醇浸提法是提取薄荷中黄酮类物质的有效方法。薄荷中总黄酮提取液对Fenton体系产生的·OH有很好的清除作用。 Objective To investigate the extraction and identification of total flavonoids in peppermint and its scavenging effect on hydroxyl free radicals. Methods The flavonoids in peppermint were extracted by ultrasonic extraction of ethanol, the contents of flavonoids were determined by spectrophotometry, and the scavenging effects of flavonoids on hydroxyl radical were tested. The results showed that the content of total flavonoids in the sample was 0.325mg · mL-1, the recovery rate was 99.53%, and its purity and yield were high. Conclusion Ultrasonic ethanol extraction is an effective method to extract flavonoids from peppermint. The extract of total flavonoids from peppermint has good scavenging effect on · OH produced by Fenton system.