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多杀菌素是由土壤放线菌刺糖多孢菌产生的次生代谢产物,主要活性物质为spinosyn A和spinosyn D,是一种新型的高效广谱大环内酯类杀虫剂,因其独特的杀虫机理而兼具生物农药的安全性与化学农药的快速性.简述了多杀菌素生物合成的分子机理,包括聚酮链的生物合成与修饰、NDP糖的合成、修饰与连接转移以及多杀菌素生物合成基因表达调控.通过对多杀菌素生物合成的限速步骤及相关基因的分析,探讨了提高多杀菌素产量与合成其新衍生物的定向遗传改造策略,并展望了其应用发展前景.
Spinosad is a secondary metabolite produced by soil actinomycetes polysaccharides Spores, the main active substances are spinosyn A and spinosyn D, is a new type of highly efficient broad-spectrum macrolide insecticide, because of its The unique insecticidal mechanism combines the safety of biological pesticides with the rapidity of chemical pesticides.The molecular mechanism of spinosad biosynthesis is briefly described, including the biosynthesis and modification of polyketide chains, the synthesis, modification and connection of NDP sugars Transfer and spinosad biosynthesis gene expression regulation.By spinosad biosynthesis rate-limiting steps and related genes analysis to explore the spinosad yield and synthesis of the new derivatives of directional genetic transformation strategy, and prospects Its application development prospects.