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目的用大气细颗粒物(PM_(2.5))染毒人正常肺上皮细胞(BEAS-2B细胞),探讨大气细颗粒物(PM_(2.5))不同成分对人正常肺上皮细胞(BEAS-2B)的细胞毒性和氧化损伤。方法采集乌鲁木齐市大气细颗粒物(PM_(2.5)),实验细胞为人正常肺上皮细胞株BEAS-2B细胞。为比较细颗粒物不同成分对细胞毒性的大小,超声震荡洗脱石英纤维滤膜中的细颗粒物,分析其水溶成分和非水溶成分的毒性。结果对PM_(2.5)中水溶成分和非水溶成分进行剂量为50μg/m L、100μg/m L、200μg/m L和300μg/m L的浓度染毒,结果显示水溶成分的细胞毒性高于非水溶成分(P<0.01)。结论大气细颗粒物(PM_(2.5))可导致细胞损伤,并且组成成分的不同可以导致其对细胞毒性的差异。
OBJECTIVE To investigate the effect of different components of atmospheric fine particles (PM_ (2.5)) on human normal lung epithelial cells (BEAS-2B) by using airborne fine particles (PM_ (2.5) Toxicity and oxidative damage. Methods The airborne particulate matter (PM_ (2.5)) in Urumqi was collected and the cells were cultured in human normal epithelial cell line BEAS-2B. In order to compare the cytotoxicity of different components of fine particles, the fine particles in the quartz fiber membrane were eluted by ultrasonic vibration and the toxicity of water-soluble and non-water-soluble components was analyzed. Results The water-soluble and water-insoluble components of PM 2.5 were exposed to a concentration of 50μg / ml, 100μg / ml, 200μg / ml and 300μg / ml respectively. The results showed that the cytotoxicity of water-soluble components was higher than that of non- Water soluble ingredients (P <0.01). Conclusions Airborne fine particulate matter (PM_ (2.5)) can lead to cell damage, and the differences in composition can lead to differences in cytotoxicity.