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目的:探讨组织蛋白酶K、S(Cat-K,Cat-S)与动脉粥样硬化及其斑块不稳定性的关系。方法:原代培养小鼠主动脉平滑肌细胞(VSMC),传代后用白细胞介素-1(IL-1β)(10μg/L)诱导细胞12、24、36h,免疫荧光检测VSMC Cat-K、Cat-S的表达变化,ELISA试剂盒检测细胞培养基Cat-K、Cat-S的浓度。结果:原代培养的细胞VSMC-a-actin阳性,细胞纯度达96以上。对照组Cat-K、Cat-S免疫荧光染色均为阴性;经IL-1β诱导后Cat-K弱阳性,而Cat-S则呈阳性,并随着诱导时间的延长荧光强度不断增强。对照组培养基Cat-K、Cat-S浓度微量;经IL-1β诱导后,Cat-K无明显增加,但Cat-S增加明显,与对照组相比,差异有统计学意义(P<0.01),24h后达到高峰并趋于稳定。结论:IL-1β可促进VSMC分泌Cat-K、Cat-S,以后者显著,提示Cat-S在动脉硬化的发生、发展及斑块不稳定性中起着重要作用。
Objective: To investigate the relationship between cathepsin K, S (Cat-K, Cat-S) and atherosclerosis and plaque instability. Methods: Mouse primary aortic smooth muscle cells (VSMCs) were cultured and subcultured for 12, 24 and 36 h with interleukin-1 (IL-1β) (10 μg / L) -S expression changes, ELISA kit detection of cell culture medium Cat-K, Cat-S concentration. Results: Primary cultured cells VSMC-a-actin positive cell purity of 96 or more. Cat-K and Cat-S immunofluorescence staining were negative in the control group; Cat-K was weakly positive and Cat-S was positive after induced by IL-1β, and the fluorescence intensity was continuously increased with the induction time. The concentration of Cat-K and Cat-S in the control group was slight, while Cat-K was not increased but the Cat-S increased obviously after induced by IL-1β, the difference was statistically significant (P <0.01 ), Reached the peak after 24h and tended to be stable. CONCLUSION: IL-1β can promote the secretion of Cat-K and Cat-S in VSMCs, which indicates that Cat-S plays an important role in the occurrence and development of atherosclerosis and plaque instability.